Data Summary and Peer Evaluations

After you submit the final draft of the Data Analysis and Conclusions assignment, you will be assigned the Data Analysis and Conclusions document from a student in the other group (by the end of the day, Tuesday, 12/8). If you are in Exercise 6 Group 1, then you will be assigned a peer document from a student in Exercise 6 group 2 and vice versa. If the document assigned to you does not have data from the other group, or has incomplete information then you must send Dr. Mohan an email (smohan12@umd.edu) requesting a new peer document assignment no later than Friday, 12/11. Due date extensions will not be granted because you waited too long to check the peer document assigned to you.

You will evaluate this peer document by filling out the rubric for the final draft of the Data Analysis and Conclusions document assigned to you by noon on Monday, 12/14. You will also use the information in the peer document assigned to you to complete the Data Summary assignment (see below for additional details), by noon on Monday, 12/14. To ensure that you are using accurate information to complete the data summary assignment an answer key for the data analysis and conclusions assignment assigned to the other group will be added to your Exercise 6 group page by Tuesday, 12/8. You should also use this answer key as a reference to complete the peer evaluation assignment.

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Refer to the instructions outlined in “How do I submit a peer review to an assignment?
” for information on how to access the peer document assigned to you and fill out the rubric. Remember, the document you are evaluating is the final draft of the Data Analysis and Conclusions assignment.

In the data summary assignment, you will use the data generated for the research questions assigned to your Exercise 6 group and the Data Analysis and Conclusions document assigned to you for peer evaluation to address the two overall goals of Exercise 6:

Determine treatment options for MH1 infections
Determine if MH1 is a strain of E. coli or salmonella.

In addition to the experiments you and your peers analyzed for the Data Analysis and Conclusions assignment, you will analyze the results from two new experiments. These experiments were carried out to confirm the results from previous experiments conducted to determine the identity of MH1.

For this assignment, you will type up the answers to the questions
listed below in a separate document, using Word or GoogleDocs. Then, convert the document to a single pdf file and submit the assignment by uploading it here by noon on Monday, 12/14. Handwritten and/or scanned files will not be graded. Additionally, you must upload the Data Analysis and Conclusions peer document that was assigned to you for evaluation.

Data Summary Assignment Questions:

List your Group number. (1pt)
Which of the antibiotics tested in this exercise (Ampicillin, Kanamycin, Tetracycline and Chloramphenicol), can be used to treat MH1 infections? Provide a rationale for your answer and indicate if your conclusion is based on results you analyzed or on results analyzed by a peer assigned to the other group. (2pts)
In 1-2 sentences summarize how MH1 is resistant to the penicillin family of antibiotics. Provide a rationale for your answer and indicate if your conclusion is based on results you analyzed or on results analyzed by a peer assigned to the other group. (2pts)
Based on the results from the beta – galactosidase assay and the GapA, ApeE, and InvA PCR experiments can you determine if MH1 is a strain of E. coli or Salmonella or is the overall conclusion about the identity of MH1 still inconclusive? Provide a rationale for your answer and indicate if your conclusion is based on results you analyzed or on results analyzed by a peer assigned to the other group. (2pts)
To confirm the results from the beta-galactosidase assay, an additional PCR experiment was carried out using primers for the beta-galactosidase gene and DNA template from MH1, E. coli and Salmonella. After gel electrophoresis, a single band was observed in the lane with the E.coli sample and in the lane with the MH1 sample.
Based on the results from the beta-galactosidase assay, are these results expected? Provide a brief rationale for your answer. (1pts)
Do these results change your conclusion about the identity of MH1? Provide a brief rationale for your answer. (2pts)
To confirm the results from the ApeE PCR experiment, the PCR product from the MH1 sample was sequenced. Follow these directions
to perform a BLAST analysis of the MH1/ApeE PCR product and then answer the questions listed below.
Based on the top five matches from the BLAST analysis, what can you conclude about the identity about the DNA fragment that was amplified in the MH1/ApeE PCR reaction? Provide a rationale for your conclusion. (2pts)
Do these results from BLAST analysis change your conclusion about the identity of MH1? Provide a brief rationale for your answer.

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