To dilute something is basically the process of reducing the concentration of a substance in a solution. Serial dilutions are used in many experimental types including biochemistry, microbiology, and pharmacology. Often solutions need to be diluted to a more useful working concentration.
Dilution Factor (DF) = Final Volume ÷ Solute Volume
The DF can be used alone or as the denominator of the fraction, for example, a DF of 20 means a 1:20 dilution, or 1-part solute + 19 parts diluent, for a total of 20 parts.
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